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Correlations of sputum eosinophil percentages with T2 gene expression in the whole cohort. Associations between eosinophil percentages and IL13 ( A ), CLCA1 ( B ), CCL26 ( C ) and CST1 ( D ) were assessed by Spearman’s rank test with Rho and p-values are presented for each analysis.

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: Decreased Sputum Type-2 Gene Expression in COPD Current Smokers

doi: 10.2147/COPD.S546528

Figure Lengend Snippet: Correlations of sputum eosinophil percentages with T2 gene expression in the whole cohort. Associations between eosinophil percentages and IL13 ( A ), CLCA1 ( B ), CCL26 ( C ) and CST1 ( D ) were assessed by Spearman’s rank test with Rho and p-values are presented for each analysis.

Article Snippet: Reverse transcription was performed on 50 ng of RNA using the Verso cDNA kit (Thermo Fisher Scientific). cDNA was reacted with ABsolute blue qPCR mix (Thermo Fisher Scientific) in 25 μL reactions containing premade ABI Taqman gene expression assays (Catalogue no: 4331182) for IL13 (Hs00174379_m), CLCA1 (Hs00976287_m1), CCL26 (Hs00171146_m1), CST1 (Hs00606961_m1), or the endogenous control glyceraldehyde-3phosphate dehydrogenase (GAPDH) (Catalogue no: 4352934E) (Life Technologies, Parsley, UK).

Techniques: Gene Expression

Repeat analysis of sputum T2 gene expression in COPD patients. Sputum samples were collected from 26 COPD patients at baseline and at 6 months. Gene expressions for IL13 ( A ), CLCA1 ( B ), CCL26 ( C ) and CST1 ( D ) were quantified by RT-PCR. Associations between baseline and 6-month measurements were assessed by Spearman’s rank test and Interclass coefficient analysis. Rho, p-values and ICC values are presented for each analysis. The number of patients with undetectable levels at both baseline and 6 months were n=2 for IL13 , n=12 for CLCA1 , n=0 for CCL26 and n=4 for CST1 .

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: Decreased Sputum Type-2 Gene Expression in COPD Current Smokers

doi: 10.2147/COPD.S546528

Figure Lengend Snippet: Repeat analysis of sputum T2 gene expression in COPD patients. Sputum samples were collected from 26 COPD patients at baseline and at 6 months. Gene expressions for IL13 ( A ), CLCA1 ( B ), CCL26 ( C ) and CST1 ( D ) were quantified by RT-PCR. Associations between baseline and 6-month measurements were assessed by Spearman’s rank test and Interclass coefficient analysis. Rho, p-values and ICC values are presented for each analysis. The number of patients with undetectable levels at both baseline and 6 months were n=2 for IL13 , n=12 for CLCA1 , n=0 for CCL26 and n=4 for CST1 .

Article Snippet: Reverse transcription was performed on 50 ng of RNA using the Verso cDNA kit (Thermo Fisher Scientific). cDNA was reacted with ABsolute blue qPCR mix (Thermo Fisher Scientific) in 25 μL reactions containing premade ABI Taqman gene expression assays (Catalogue no: 4331182) for IL13 (Hs00174379_m), CLCA1 (Hs00976287_m1), CCL26 (Hs00171146_m1), CST1 (Hs00606961_m1), or the endogenous control glyceraldehyde-3phosphate dehydrogenase (GAPDH) (Catalogue no: 4352934E) (Life Technologies, Parsley, UK).

Techniques: Gene Expression, Reverse Transcription Polymerase Chain Reaction